{"id":2321,"date":"2025-06-04T19:14:01","date_gmt":"2025-06-04T16:14:01","guid":{"rendered":"https:\/\/sonerduzguner.com\/?p=2321"},"modified":"2026-10-26T14:49:09","modified_gmt":"2026-10-26T11:49:09","slug":"microinjection-and-embryo-development-monitoring","status":"publish","type":"post","link":"https:\/\/www.sonerduzguner.com\/en\/microinjection-and-embryo-development-monitoring\/","title":{"rendered":"Microinjection and Embryo Development Monitoring"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><div      class=\"vc_row wpb_row section vc_row-fluid\" style=' padding-bottom:40px; text-align:left;'><div class=\"full_section_inner clearfix\"><div class=\"wpb_column vc_column_container vc_col-sm-12\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\">\n\t<div class=\"wpb_text_column wpb_content_element\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<p>Mikroenjeksiyon (ICSI), spermin mikroskop alt\u0131nda do\u011frudan yumurtan\u0131n i\u00e7ine enjekte edildi\u011fi bir yard\u0131mc\u0131 \u00fcreme tekni\u011fidir. D\u00f6llenmenin ard\u0131ndan embriyolar\u0131n geli\u015fimi laboratuvarda yak\u0131ndan takip edilerek transfer i\u00e7in en uygun embriyolar belirlenir. Bu yaz\u0131da mikroenjeksiyon y\u00f6ntemini ve embriyo geli\u015fim takibini bulabilir, Op. Dr. Soner D\u00fczg\u00fcner\u2019in konuyu anlatt\u0131\u011f\u0131 videoyu izleyebilirsiniz.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n\t<div class=\"wpb_text_column wpb_content_element\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<h2><span class=\"ez-toc-section\" id=\"Mikroenjeksiyon_Nedir\"><\/span>What is Microinjection?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Microinjection is an assisted reproductive technique specially developed for treating severe male infertility. This method aims to achieve fertilization by directly injecting the sperm into the egg when the sperm lacks normal fertilization capability. IVF and other known treatment methods have low or no fertilization rates in cases of severe male infertility. In cases of low sperm count, motility, or morphological abnormalities, natural fertilization of the egg by sperm is not possible. In microinjection, under a microscope, sperm is injected into the egg retrieved from the female using micro-pipettes. The procedures are performed under the microscope with 200-400x magnification on a heated surface using a micromanipulator.<\/p>\n\n\t\t<\/div>\n\t<\/div><div class=\"vc_empty_space\"  style=\"height: 20px\" >\n\t<span class=\"vc_empty_space_inner\">\n\t\t<span class=\"empty_space_image\"  ><\/span>\n\t<\/span>\n<\/div>\n\t<div class=\"wpb_raw_code wpb_raw_html wpb_content_element\" >\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<iframe class=\"icerik-video\" width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/S3PQzEu3Dzg?si=aGKeS3vPKEUoNn8j\" title=\"YouTube video player\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n\t\t<\/div>\n\t<\/div>\n<div class=\"vc_empty_space\"  style=\"height: 15px\" >\n\t<span class=\"vc_empty_space_inner\">\n\t\t<span class=\"empty_space_image\"  ><\/span>\n\t<\/span>\n<\/div>\n\t<div class=\"wpb_text_column wpb_content_element\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<p style=\"text-align: center;\"><strong><em>Bu videoda sperm mikroenjeksiyonu (mikroenjeksiyon) y\u00f6ntemi ve embriyo geli\u015fim s\u00fcrecinin nas\u0131l takip edildi\u011fi hakk\u0131nda bilgi bulabilirsiniz<\/em><\/strong><\/p>\n\n\t\t<\/div>\n\t<\/div><\/div><\/div><\/div><\/div><\/div><div      class=\"vc_row wpb_row section vc_row-fluid\" style=' text-align:left;'><div class=\"full_section_inner clearfix\"><div class=\"blog-detay wpb_column vc_column_container vc_col-sm-12\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\">\n\t<div class=\"wpb_text_column wpb_content_element\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<h2><span class=\"ez-toc-section\" id=\"Mikroenjeksiyon_Yontemi_Kimlere_Uygulanir\"><\/span>Who is Eligible for the Microinjection Method?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The microinjection method can also be applied to couples where fertilization cannot be achieved with IVF or where there is an insufficient number and\/or quality of sperm and eggs for IVF. The procedure can be performed using semen with very low sperm count or, in cases where no sperm are found in the semen, sperm obtained from the testes can be used. In some men, sperm may not be found in the testes. In such cases, if available, spermatids, which are immature cells that develop into sperm, can be used. However, results obtained using spermatids are not yet fully successful, and fertilization rates are low.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Mikroenjeksiyon_Nasil_Yapilir\"><\/span>How is Microinjection Performed?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The microinjection procedure is performed under a microscope equipped with specialized equipment, using sterile, single-use glass micropipettes. The eggs obtained after the egg retrieval procedure are cleaned of surrounding cumulus cells to identify mature and usable eggs. A special culture medium containing sperm from the male is prepared in a sterile, non-toxic plastic dish for the microinjection process. Then, microdroplets containing special culture medium for the mature eggs are prepared. The sperm is immobilized by pressing its midpiece with the microinjection pipette. The immobilized sperm is then aspirated into the pipette and injected into the droplet containing the egg. This procedure is applied sequentially to all eggs. After completion, the eggs are transferred to special culture media, and fertilization is checked 18-20 hours later.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Mikroenjeksiyon_Hangi_Durumlarda_Uygulanir\"><\/span>In which cases is Microinjection applied?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>In cases of severe male infertility, sperm lack the ability to fertilize the egg. Severe male infertility is defined as having fewer than one million sperm with rapid progressive motility and normal morphology. Even when both sperm and egg are completely normal, fertilization may not occur with the IVF method. In cases of unexplained infertility, fertilization fails in 15-20% of IVF attempts. If IVF is applied directly to couples with normal sperm parameters, fertilization failure may occur. Therefore, in couples with a sufficient number of eggs (10 or more), microinjection and IVF methods are used together. This way, eggs are divided into two groups: fertilization is checked by IVF, and microinjection is used as a precaution against fertilization failure.<\/p>\n<p>Although ICSI offers a promising treatment option for male infertility today, the most important factors affecting success are the woman's age and the quality of the retrieved eggs. Factors caused by advanced maternal age and increased chromosomal abnormalities influence the success of male infertility treatment depending on the woman.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Embriyo_Gelisim_Takibi_Nedir_ve_Nasil_Yapilmaktadir\"><\/span>What is Embryo Development Monitoring and How Is It Performed?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Ensuring embryo development in IVF treatment is the most important step in laboratory conditions. After fertilization, embryo development is carefully monitored under laboratory conditions and with the guidance of an experienced team, and the transfer procedure is performed at the appropriate time.<\/p>\n<p class=\"translation-block\"><strong>Fertilization:<\/strong> Fertilization refers to the fusion of a sperm cell with an egg cell. This process occurs when spermatozoa penetrate the outer membrane of the egg and reach the cytoplasm of the egg cell. As a result of fertilization, a single-celled embryo called a zygote is formed.<\/p>\n<p class=\"translation-block\"><strong>Cellular Division:<\/strong> The zygote divides to form more cells. First, the zygote divides into a two-cell embryo, and then cell divisions continue. These divisions result in a multicellular embryo. The first division usually occurs about 24 hours later, followed by cell divisions at regular intervals.<\/p>\n<p class=\"translation-block\"><strong>Morphological Changes:<\/strong> The embryo undergoes morphological changes alongside cellular divisions. A regular arrangement forms between the cells, and the embryo divides into groups of cells called blastomeres. At the same time, the embryo becomes a morula, composed of small cells formed by mitotic divisions.<\/p>\n<p class=\"translation-block\"><strong>Blastocyst Formation:<\/strong> In the later stages of early embryo development, the morula structure transforms into a blastocyst. The blastocyst contains an inner cell mass (ICM) and a layer of cells called the trophoblast. The inner cell mass serves as the source of cells that will form various organs and tissues of the embryo in later stages, while the trophoblast will develop into cells that form the placenta and supportive tissues during early pregnancy.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Mikroenjeksiyon_Yontemi_mi_Tup_Bebek_mi\"><\/span>Microinjection Method or IVF?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>In the microinjection method, the chances of pregnancy and fertilization are higher compared to the classic IVF method. However, success rates vary depending on the condition of the couple.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Mikroenjeksiyon_Enjeksiyon_Ne_Zaman_Yapilir\"><\/span>When is the Microinjection Injection Performed?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The microinjection method is performed after the eggs are prepared following the egg retrieval stage.<\/p>\n\n\t\t<\/div>\n\t<\/div><\/div><\/div><\/div><\/div><\/div><div      class=\"vc_row wpb_row section vc_row-fluid  sizi-arayalim vc_custom_1748263515128\" style=' text-align:left;'><div class=\"full_section_inner clearfix\"><div class=\"wpb_column vc_column_container vc_col-sm-12\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div      class=\"vc_row wpb_row section vc_row-fluid vc_inner  vc_custom_1748108924602\" style=' text-align:left;'><div class=\"full_section_inner clearfix\"><div class=\"wpb_column vc_column_container vc_col-sm-12\"><div class=\"vc_column-inner vc_custom_1746548174605\"><div class=\"wpb_wrapper\">\n\t<div class=\"wpb_text_column wpb_content_element\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<div style=\"color: #4c4c4c; 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Dr. Soner D\u00dcZG\u00dcNER<\/h4>\n\n\t\t<\/div>\n\t<\/div>\n\t<div class=\"wpb_text_column wpb_content_element  blog-detay-unvan\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<p>Obstetrics and Gynaecology Specialist<\/p>\n\n\t\t<\/div>\n\t<\/div><div class=\"vc_empty_space\"  style=\"height: 5px\" >\n\t<span class=\"vc_empty_space_inner\">\n\t\t<span class=\"empty_space_image\"  ><\/span>\n\t<\/span>\n<\/div>\n\t<div class=\"wpb_text_column wpb_content_element  blog-detay-unvan-desc\">\n\t\t<div class=\"wpb_wrapper\">\n\t\t\t<p>Op. Dr. Soner D\u00fczg\u00fcner: Provides diagnosis and treatment in areas such as in vitro fertilization, women's health, infertility, gynecological surgery and pregnancy follow-up.<\/p>\n\n\t\t<\/div>\n\t<\/div><div class=\"vc_empty_space\"  style=\"height: 15px\" >\n\t<span class=\"vc_empty_space_inner\">\n\t\t<span class=\"empty_space_image\"  ><\/span>\n\t<\/span>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Oocyte vitrification, women having a limited number of oocyte reserves and oocyte quality<\/p>","protected":false},"author":1,"featured_media":2392,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16],"tags":[],"class_list":["post-2321","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tedaviler"],"_links":{"self":[{"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/posts\/2321","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/comments?post=2321"}],"version-history":[{"count":11,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/posts\/2321\/revisions"}],"predecessor-version":[{"id":4441,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/posts\/2321\/revisions\/4441"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/media\/2392"}],"wp:attachment":[{"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/media?parent=2321"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/categories?post=2321"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sonerduzguner.com\/en\/wp-json\/wp\/v2\/tags?post=2321"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}